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| Flow Sorters |
Flow Analysers |
Analysis Computers |
Analysis Software |
Education & Training |
Troubleshooting |
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FlowCore currently has three cell sorters operating within the facility. A summary chart of their capabilities is presented below.
Not sure which sorter you should book? Click here for useful guide.
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Influx 1
(5 laser) |
Influx 2
(3 laser) |
FACSVantage-Diva
(3 laser) |
| Sample Tube Compatibility |
"Soft" FACS tubes
(5ml polypropylene round-bottom tube) |
"Soft" FACS tubes
(5ml polypropylene round-bottom tube) |
"Hard" FACS tubes
(5ml polystyrene round-bottom tube) |
| Max. Sample Rate |
50,000 events/sec |
50,000 events/sec |
30,000 events/sec |
| Available Nozzle Setups1 |
70um @ 60psi |
100um @ 20psi |
70um @ 60psi |
100um @ 20psi |
80um @ 30psi |
100um @12psi |
| Corresponding Maximum Sorting Rate |
25,000 events/sec |
10,000 events/sec |
25,000 events/sec |
10,000 events/sec |
10,000 events/sec |
6,500 events/sec |
| Alternate Nozzle Setups |
Contact a FlowCore staff member if you require a different nozzle setup. |
488nm Blue Laser
(FITC, A488, GFP, CFSE, PE+tandems, PI, 7AAD) |
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633-640nm Red Laser
(APC+tandems, A647, A680, A700) |
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561nm Yellow Laser2
(PE+tandems, RFP, DsRed, mCherry, A568, A594) |
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350nm UV Laser
(DAPI, Hoechst) |
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405nm Violet Laser
(Pacific Blue, Pacific Orange, Qdots) |
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| Sterile Sorting |
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| Sorting of Infectious Material |
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| Tissue Culture Plate Deposition (inc. single cell) |
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1Generally we recommend smaller nozzles for lymphocytes & stem cells, and larger nozzles for cultured cells & larger particles (eg stromal cells, megakaryocytes, protoplasts).
Please contact us if you are unsure which nozzle to choose.
2The yellow laser line excites PE more optimally, and eliminates spillover issues with bright GFP signals. |
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