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| Flow Sorters |
Flow Analysers |
Analysis Computers |
Analysis Software |
Education & Training |
Troubleshooting |
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FlowCore currently has three cell sorters operating within the facility. A summary chart of their capabilities is presented below.
Not sure which sorter you should book? Click here for useful guide.
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Influx 1
(5 laser) |
Influx 2
(3 laser) |
FACSVantage
DiVa |
| Sample Tube Compatibility |
"Soft" FACS tubes
(5ml polypropylene round-bottom tube) |
"Soft" FACS tubes
(5ml polypropylene round-bottom tube) |
"Hard" FACS tubes
(5ml polystyrene round-bottom tube) |
| Max. Sample Rate |
50,000 events/sec |
50,000 events/sec |
30,000 events/sec |
| Standard Nozzle Setup |
90um @ 30psi |
70um @ 60psi |
80um @ 30psi |
| Standard Max. Sort Rate |
12,000 events/sec |
25,000 events/sec |
10,000 events/sec |
| Alternate Nozzle Setups |
Contact a FlowCore staff member for more information. |
488nm Blue Laser
(FITC, A488, GFP, CFSE, PE+tandems, PI, 7AAD) |
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633-640nm Red Laser
(APC+tandems, A647, A680, A700) |
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561nm Yellow Laser1
(PE+tandems, RFP, DsRed, mCherry, A568, A594) |
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350nm UV Laser
(DAPI, Hoechst) |
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405nm Violet Laser
(Pacific Blue, Pacific Orange, Qdots) |
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| Sterile Sorting |
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| Sorting of Infectious Material |
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| Tissue Culture Plate Deposition (inc. single cell )2 |
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1The yellow laser line excites PE more optimally, and eliminates spillover issues with bright GFP signals.
2Whilst plate sorting is possible on all machines, it is most easily performed using the DiVa software. |
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