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| Flow Sorters |
Flow Analysers |
Analysis Computers |
Analysis Software |
Education & Training |
Troubleshooting |
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FlowCore currently has one flow cytometric analyser operating within the facility. A summary chart of its capabilities is presented below.
Free training is provided for the analyser with the first booking, after which the analysis machine is typically run without assistance. If you would like to book a training session or require extra help running your samples, please contact us to organise a time with a staff member before making your booking.
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LSRIIa
(4 Lasers) |
LSRIIb
(7 Lasers) |
| Sample Tube Compatibility |
"Hard" FACS tubes
(5ml polystyrene round-bottom tube) |
"Hard" FACS tubes
(5ml polystyrene round-bottom tube) |
| Max. Sample Rate |
30,000 events/sec |
30,000 events/sec |
355nm UV Laser
(Hoechst) |
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405nm Violet Laser
(DAPI, Pacific Blue, Pacific Orange, Qdots) |
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488nm Blue Laser
(FITC, A488, GFP, CFSE, PE+tandems, PI) |
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532nm Green Laser
(A555) |
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561nm Yellow Laser1
(PE+tandems, RFP, DsRed, mCherry, A568) |
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592nm Orange Laser
(A594) |
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628-640nm Red Laser
(APC+tandems, A647, A680, A700) |
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High-throughput Plate Sampler
(96 well, 384 well)2 |
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1The yellow laser line excites PE more optimally, and eliminates spillover issues with bright GFP signals.
2To ensure compatibility with the LSRII plate loader, please only use plates manufactured by BD Bioscience (Falcon). |
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