FlowCore: A world-class flow cytometry facility servicing Monash University and the broader scientific community of Melbourne Core Flow Cytometry Facility
Home The Facility
Equipment & Services
Data Management Policy Useful Info & FAQs Hours & Rates Bookings & Access Contact Us
Useful Infomation & Links FAQs
 
How much time should I book?
 

Unfortunately this is akin to asking "How long is a piece of string?" - it depends on the concentration and volume of your cell suspension and, for the cell sorters, the nozzle size and sheath pressure.

Your best bet is to consult a FlowCore staff member and be generous with your booking time if you're running an assay for the first time, but as a general guide:

Cell sorters
For small cell types (blood cells etc.) we can run up to 25,000 cells through the machine per second, assuming your sample is sufficiently concentrated. For larger cell types (cell lines, digested tissue) the maximum event rate will be closer to 6-8,000 cells per second.

Analysers
The LSRII is capable of running cells at up to 30,000 cells per second - again, this depends on your sample being concentrated enough.

And don't forget to take into consideration the time it takes to change tubes ... this can take much longer than you think if you have a lot of samples!

Please also note the standard nozzle setups for the cell sorters - if you are requesting a non-standard setup, you must allow 15 minutes setup time in your booking.

 

Back to FAQs
 
Monash University